2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

2-Nitrophenyl-beta-D-glucopyranoside o se vailaʻau vailaʻau e aofia ai se mole mole glucopyranoside faʻapipiʻi i se vaega nitrophenyl.E masani ona faʻaaogaina e fai ma sui i suʻega enzymatic e iloa ma faʻatusatusa le gaioiga o enzymes pei ole beta-glucosidase.O le vaega o le nitrophenyl e mafai ona vavaeina e le enzyme, e mafua ai le tuʻuina atu o se oloa lanu samasama e mafai ona fuaina spectrophotometrically.O lenei tuufaatasiga e sili ona aoga i le suʻesuʻeina o enzyme kinetics ma suʻesuʻega maualuga o le enzyme inhibitors poʻo le activators.O lo'o fa'afaigaluegaina fo'i i su'esu'ega fa'aolaola mo le su'esu'eina o le metabolism o le ga'o ma e pei o se mea e fa'atatau i le glycosidic-linkage-specific substrate.

Poloaiga po'o Talosaga Fa'amatalaga

Fa'amatalaga Oloa

Faailoga o oloa

Fa'aoga ma A'afiaga

Enzyme substrate: ONPG e masani ona faʻaaogaina e fai ma sui mo beta-galactosidase, o se enzyme e hydrolyzes ONPG e maua ai se mea lanu samasama (o-nitrophenol) e faigofie ona iloa spectrophotometrically.E mafai ona fa'aogaina lenei tali fa'a-enzymatic e fua ai le gaioiga o le beta-galactosidase, ma avea ai le ONPG ma meafaigaluega taua i su'esu'ega enzymology.

Su'esu'ega mole biology: O lo'o fa'aogaina le ONPG e fai ma sui i su'esu'ega o meaola mole mole eseese, aemaise ile su'esu'ega o gene a le fai lipoti o le beta-galactosidase.I nei suʻesuʻega, e faʻaaogaina se substrate faʻavae ONPG e fua ai le gaioiga o le gene reporter, lea e masani ona faʻatonutonuina e se faʻasologa faʻasalalauga faʻapitoa o tului.O le gaioiga beta-galactosidase, faʻaalia e le suiga o lanu na gaosia i luga ole ONPG hydrolysis, e maua ai faʻamatalaga taua e uiga i le gaioiga faʻalauiloa.

Su'esu'ega o fa'amatalaga o kenera: O lo'o fa'aaogaina fo'i le ONPG i le su'esu'eina o fa'amatalaga o kenera.E ala i le faʻafesoʻotaʻi o le faʻasologa o le fiafia i le gene beta-galactosidase, e mafai e tagata suʻesuʻe ona fuaina le gaioiga beta-galactosidase e faʻaaoga ai le ONPG e fai ma sui.Ole maualuga ole gaioiga ole beta-galactosidase e atagia mai ai le malosi ma le gaioiga a le tagata faʻalauiloa, faʻatagaina mo le iloiloga o tulaga faʻaalia o gene.

Talosaga mo su'esu'ega: E mafai ona fa'aogaina le ONPG i fa'ata'ita'iga fa'ata'ita'iga, aemaise ile su'esu'eina o meaola ninii fa'ama'i.O siama eseese, e pei ole Escherichia coli ma nisi ituaiga o Shigella ma Salmonella, e maua mai ai le beta-galactosidase e mafai ona pipii ONPG.Ole tali ole hydrolysis lea e maua ai se suiga o lanu vaaia, lea e mafai ona fa'aoga e iloa ai le iai o nei siama ile fa'ata'ita'iga ile falema'i.

Fa'ata'ita'iga o oloa

Fa'aputuga o oloa:

Fa'amatalaga Faaopoopo:

Tulaga	C12H15NO8
Su'ega	99%
Fa'aaliga	Pa'u samasama mama
CAS Nu.	2816-24-2
afifiina	Laiti ma tele
Ola Fa'asa	2 tausaga
Teuina	Teu i se nofoaga malulu ma mago
Fa'ailoga	ISO.