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N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium masima CAS:66992-27-6
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, lea e taʻua foi o HEPES sodium salt, o se vailaʻau vailaʻau e masani ona faʻaaogaina e fai ma sui o le pH i totonu o fale suʻesuʻe meaola ma vailaʻau.E fesoasoani e fa'amautu se tulaga mautu o le pH, fa'apena lelei mo fa'aoga e pei o le aganu'u fa'aola, su'esu'ega enzyme, su'esu'ega porotini, electrophoresis, ma vaila'au.HEPES sodium masima e fa'amautinoa tulaga sili ona lelei mo fa'agaioiga olaola ma fa'aleleia le sa'o ma le fa'amaoni o fa'ai'uga fa'ata'ita'iga.
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S-Butyrylthiocholine iodide CAS:1866-16-6
S-Butyrylthiocholine iodide o se vailaʻau vailaʻau e masani ona faʻaaogaina i suʻesuʻega faʻapitoa ma enzymatic.O se mea e fai mo le enzyme butyrylcholinesterase (BChE) ma e faʻaaogaina e fua ai lona gaioiga.
A fa'asusu le S-Butyrylthiocholine iodide e le BChE, e maua mai ai le thiocholine ma le butyric acid e fai ma oloa.O le tuʻuina atu o le thiocholine e mafai ona fuaina i le faʻaogaina o le spectrophotometric poʻo le fluorometric assay, faʻatagaina le faʻatusatusaina o gaioiga BChE.
S-Butyrylthiocholine iodide e masani ona faʻaaogaina i falemaʻi ma suʻesuʻega faʻapitoa e iloilo ai le gaioiga o le BChE i faʻataʻitaʻiga e pei o le plasma toto poʻo le masini.E mafai ona faʻaaogaina e iloilo ai le galuega enzymatic o le BChE ma lona tulaga gafatia i le tele o gaioiga faʻapitoa, faʻapea foʻi ma le suʻesuʻeina ma le mataʻituina o nisi tulaga faʻafomaʻi.
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ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium masima) CAS:30931-67-0
Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), e masani ona taʻua o le ABTS, o se mea e masani ona faʻaaogaina chromogenic substrate i suʻesuʻega o meaola, aemaise lava ile vaega ole enzymology.O se mea faʻapipiʻi e faʻaaogaina e fua ai le gaioiga o enzymes eseese, e aofia ai peroxidases ma oxidases.
O le ABTS e leai se lanu i lona fa'ama'i fa'ama'i ae e liu lanumoana-lanumeamata pe a fa'ama'iina e se enzyme pe a iai le hydrogen peroxide po'o le okesene mole.O lenei suiga lanu e mafua ona o le faʻavaeina o se cation radical, lea e mitiia le malamalama i le fusi o alaleo.
Ole tali ile va ole ABTS ma le enzyme e maua mai ai se oloa lanu e mafai ona fua ile spectrophotometrically.O le malosi o le lanu e fa'atatau tonu i le gaioiga enzymatic, e mafai ai e tagata su'esu'e su'esu'e ona iloilo fa'atusatusaga o le enzyme kinetics, enzyme inhibition, po'o fegalegaleaiga enzyme-substrate.
ABTS o lo'o i ai le tele o fa'aoga i vaega eseese, e aofia ai su'esu'ega fa'afoma'i, su'esu'ega fa'afoma'i, ma su'esu'ega o mea'ai.E sili ona maaleale ma ofoina atu le tele o le faʻaogaina o mea, ma avea ai ma filifiliga taʻutaʻua mo le tele o suʻega biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
4-Nitrophenyl α-D-maltohexaoside o se faʻapipiʻi e aofia i le vasega o fesoʻotaʻiga α-glycosidic.O se mea e maua mai i le maltose, o se disaccharide e fausia i iunite kulukose e lua.I totonu o lenei tuufaatasiga, o le vaega hydroxyl o le kulukose muamua iunite ua suia i se vaega nitrophenyl.
O lenei tuufaatasiga e masani ona faʻaaogaina e fai ma sui i suʻesuʻega enzymatic e suʻesuʻe ai le gaioiga o enzymes eseese, aemaise lava i latou o loʻo aʻafia i le metabolism carbohydrate.O le vaega o le nitrophenyl e mafai ai ona faigofie ona suʻesuʻeina ma faʻatusatusaina faʻamaʻi faʻamaʻi e ala i le fuaina o le faʻafefe poʻo le fluorescence o le oloa tipi.
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PIPES CAS:5625-37-6 Tau Faumea
PIPES (piperazine-1,4-bisethanesulfonic acid) o se zwitterionic buffering compound e masani ona faʻaaogaina i suʻesuʻega o meaola ma meaola.Ose fa'amautu lelei pH ma se gafatia maualuga mo le fa'atumauina tulaga fa'amautu o le pH i le pH va'aiga o le 6.1 i le 7.5.O PIPES e itiiti le fa'alavelave i biomolecules ma e talafeagai mo su'ega fa'alagolago i le vevela.E masani ona faʻaaogaina i gel electrophoresis techniques ma vailaʻau faʻapitoa e fai ma sui faʻamautu.Ile aotelega, o le PIPES ose fa'aogaina tele ma fa'aaogaina lautele i fa'ata'ita'iga fa'apitoa.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
3,3′,5,5′-Tetramethylbenzidine, e taʻua foʻi o le TMB, o se vailaʻau vailaʻau e masani ona faʻaaogaina e fai ma mea faʻapipiʻi chromogenic i suʻega immunosorbent enzyme-linked (ELISA) ma isi suʻesuʻega faʻaola.E masani ona faʻaaogaina e iloa ai ma faʻatusatusaina le iai o enzymes e pei o le horseradish peroxidase (HRP) i faʻataʻitaʻiga olaola eseese.TMB e o'o i se suiga lanu mai le le lanu i le lanumoana pe a iai nei enzymes.Mulimuli ane, e mafai ona taofi le tali e ala i le faʻaopopoina o se acid e liua ai le lanu lanumoana i se lanu samasama mulimuli.O le malosi o le lanu samasama e faʻatatau i le aofaʻi o le enzyme o loʻo i ai, e mafai ai ona faʻatusatusa.
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APS-5 CAS:193884-53-6 Tau Faumea
(4-Chlorophenyl) thio-methanol 1-(dihydrogen phosphate) disodium salt (1: 2) o se vailaʻau faʻamaʻi e patino ile vasega ole acridine derivatives.E aofia ai le 10-methylacridine ring system ma se vaega thioether faʻapipiʻi i le tulaga 4-chlorophenyl.O lo'o iai fo'i le vaega o le methanol ma vaega phosphate e lua o lo'o fa'a'ese'ese vaega e ion sodium.
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5-Bromo-4-chloro-3-indolyl phosphate disodium masima CAS:102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) o se vailaʻau vailaʻau e masani ona faʻaaogaina i le molecular biology ma biochemistry applications.O se mea'ai chromogenic mo enzymes phosphatase alkaline.
O le BCIP e masani ona faʻaogaina faʻatasi ma le nitroblue tetrazolium (NBT) e fai ma mea e suʻe ai le gaioiga o le alkaline phosphatase.Pe a fa'a'ese'ese le BCIP e le alkaline phosphatase, e fa'atupu se pa'u lanumoana, e mafai ai ona fa'aalia le i ai o le enzyme po'o le gaioiga.
E sili ona aoga lenei fa'aoga i fa'aoga e pei ole immunohistochemistry, in situ hybridization, ma enzyme-linked immunosorbent assays (ELISAs) e iloa ai le iai po'o le fa'aogaina o biomolecules patino po'o nucleic acids.O le pa'epa'e lanumoana na faia e le BCIP e maua ai se fa'ailoga va'aia e fesoasoani i le fa'ailoaina ma le au'ili'iliga o molela'au fa'atatau i fa'ata'ita'iga fa'ata'ita'iga.
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Masima masima masima CAS:139-41-3 Tau Faumea
N,N-Bis(2-hydroxyethyl)glycine sodium salt ose vaila'au fa'aogaina e fai ma sui fa'apolopolo i le tele o fa'aoga o meaola fa'aola ma biophysical.E fesoasoani e fa'amautu le maualuga o le pH i tulaga fa'ata'ita'i, e fa'aoga ai i su'esu'ega enzyme, su'esu'ega porotini, aganu'u sela, ma faiga fa'apolofesa i Sisifo.
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4-Aminophthalhydrazide AMPPD CAS:3682-14-2
4-Aminophthalhydrazide, e taʻua foʻi o le 4-APhH, o se vailaʻau faʻamaʻi ma le fua mole mole C8H8N2O.E a'afia i le vasega o hydrazide compounds ma e maua mai le phthalic acid.
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N-Acetyl-L-cysteine CAS:616-91-1
O le N-Acetyl-L-cysteine (NAC) o se faʻasologa o le amino acid cysteine.E maua ai se puna o le cysteine ma e mafai ona faigofie ona liua i le tripeptide glutathione, o se antioxidant malosi i le tino.NAC ua lauiloa mo ona antioxidant ma mucolytic meatotino, faʻaaogaina i le tele o faʻaoga faʻalesoifua maloloina.
I le avea ai o se antioxidant, NAC fesoasoani e puipuia sela mai faʻaleagaina e mafua mai i faʻamaʻi saoloto, ituaiga okesene faʻafoʻi, ma toxins.E lagolagoina fo'i le glutathione synthesis, lea e iai sona sao taua i le fa'amamaina o le tino ma le fa'atumauina o le soifua maloloina o le tino.
Na suʻesuʻeina le NAC mo ona aoga aoga i le soifua maloloina o le manava, aemaise lava mo tagata taʻitoʻatasi e iai tulaga e pei o le bronchitis tumau, COPD, ma le cystic fibrosis.E masani ona faʻaaogaina e fai ma faʻamaʻi e fesoasoani i le manifinifi ma le faʻamavaeina o le mucus, e faigofie ai ona faʻamama le auala.
E le gata i lea, ua faʻaalia e le NAC le folafolaga i le lagolagoina o le soifua maloloina o le ate e ala i le fesoasoani i le aveeseina o mea oona, e pei o le acetaminophen, o se faʻamaʻi masani tiga.E ono iai fo'i a'afiaga puipuia mai le fa'aleagaina o le ate e mafua mai i le taumafaina o le 'ava malosi.
I le faaopoopo atu i lona antioxidant ma le manava lagolago meatotino, NAC ua suʻesuʻeina mo ona aoga aoga i le soifua maloloina o le mafaufau.O nisi suʻesuʻega e faʻaalia ai e ono iai se aafiaga lelei i faʻafitauli o le mafaufau, e pei o le atuatuvale ma le obsessive-compulsive disorder (OCD).
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Acetylthiocholine iodide CAS:1866-15-5
Acetylthiocholine iodide o se vailaʻau vailaʻau e masani ona faʻaaogaina e fai ma sui i suʻega enzyme e fua ai le gaioiga o le enzyme acetylcholinesterase (AChE).O le AChE o se enzyme e faʻafefeteina ai le neurotransmitter acetylcholine, o se laasaga taua i le faʻamutaina o le faʻasalalauga i le va o sela neura.
A faʻaaogaina e le AChE le acetylthiocholine iodide, e aveese le vaega acetyl, e mafua ai le faʻavaeina o le thiocholine ma le acetate ions.Ona tali atu lea o le Thiocholine i se mea e leai ni lanu e ta'ua o le DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) e maua ai se tuufaatasiga lanu samasama e ta'ua o le 5-thio-2-nitrobenzoate, lea e mafai ona fua spectrophotometrically.Ole fua ole atina'e lanu e fa'atatau tonu ile gaioiga ole AChE ile fa'ata'ita'iga.
