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PNPG CAS:3150-24-1 Tau Faumea
PNPG, poʻo le p-nitrophenyl β-D-glucopyranoside, o se mea laʻititi mole mole e masani ona faʻaaogaina i suʻesuʻega o meaola e fua ai le gaioiga o enzymes glucosidase.E leai se lanu ma e le faʻafefe, ae i luga o le hydrolysis e glucosidase, e liua i le p-nitrophenol, lea e iai le lanu samasama ma e faigofie ona iloa spectrophotometrically.
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ONPG CAS:369-07-3 Tau Faumea
O-nitrophenyl-β-D-galactopyranoside (ONPG) o se mea faʻapipiʻi faʻaogaina o loʻo faʻaaogaina i suʻesuʻega o meaola faʻaola ma molecular biology e fua ai le gaioiga o le enzyme β-galactosidase.E masani ona faʻaaogaina i suʻesuʻega e iloa ai le faʻaalia o le gene i totonu o siama siama, e pei o le Escherichia coli.ONPG o se mea e leai ni lanu e vavaeina e le β-galactosidase, e mafua ai le tuʻuina atu o se lanu samasama, o-nitrophenol.O le lanu samasama e mafai ona fuaina spectrophotometrically, e maua ai se fua le tuusao o le enzyme activity.O le suʻega e faʻaaoga ai le ONPG e masani ona taʻua o le ONPG assay ma o se auala faʻaaoga lautele i suʻesuʻega o meaola mole mole e iloilo ai le maualuga o faʻamatalaga o kenera e pulea e le lac operon i sela siama.
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Nitrotetrazolium Blue Chloride CAS:298-83-9
O le Nitrotetrazolium Blue Chloride (NBT) o se fa'ailoga redox e masani ona fa'aogaina i su'esu'ega fa'aletino ma biochemical.O se pauta samasama sesega e liu lanumoana pe a faʻaitiitia, e faʻaaogaina mo le iloa o le iai o nisi enzymes ma gaioiga metabolic.
E tali atu le NBT ma ave eletise ma enzymes e pei o dehydrogenases, lea o loʻo aʻafia i gaioiga eseese.A faʻaitiitia le NBT e nei enzymes, e faʻatupuina se paʻu formazan lanumoana, e mafai ai ona maua le vaʻaia poʻo le spectrophotometric.
O lenei reagent e masani ona faʻaaogaina i suʻega e pei o le NBT faʻaitiitia suʻega, lea e faʻaaogaina e iloilo ai le gaioiga o le manava o sela puipuia.E mafai foʻi ona faʻaaogaina e suʻesuʻe ai gaioiga o le enzyme ma auala faʻasolosolo i suʻesuʻega e faʻatatau i le faʻamaʻi faʻamaʻi, faʻaogaina o le cell, ma le eseesega o le cell.
Ua maua e le NBT talosaga i vaega eseese, e aofia ai microbiology, immunology, ma cell biology.E fa'apitoa, mautu, ma faigofie ona fa'aoga, ma avea ai ma filifiliga lauiloa mo le tele o fa'ata'ita'iga fa'ata'ita'iga.
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Neocuproine CAS: 484-11-7 Tau Faumea
Neocuproine o se sui chelating e masani ona faʻaaogaina i talosaga eseese, e aofia ai suʻesuʻega kemisi ma vailaʻau.E i ai se sootaga malosi mo ion apamemea ma fausia faʻatasi ma i latou.O lenei meatotino e aoga ai le neocuproine mo le suʻeina ma le faʻatusatusaina o le kopa i fofo poʻo faʻataʻitaʻiga.E le gata i lea, o le neocuproine na suʻesuʻeina mo ana faʻaoga faʻapitoa, aemaise i le togafitia o le kanesa ma faʻamaʻi neurodegenerative.
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IPTG CAS:367-93-1 Tau Faumea
Isopropyl β-D-1-thiogalactopyranoside (IPTG) o se analog synthetic o lactose lea e masani ona faʻaaogaina i suʻesuʻega o meaola mole mole ma faʻaoga biotechnology.O le IPTG e masani ona faʻaaogaina e faʻaosofia ai le faʻaalia o kenera i siama siama, lea e avea o se molecular faʻaosofia e amata ai le faʻaliliuga o kenera taulaʻi.
Pe a faʻaopoopo i le faʻalauteleina o le tuputupu aʻe, o le IPTG e faʻaaogaina e siama ma e mafai ona fusifusia i le lac repressor protein, e taofia ai mai le taofia o le gaioiga a le lac operon.O le lac operon o se fuifui o kenera o loʻo aʻafia i le lactose metabolism, ma a aveese le polotini repressor, e faʻaalia ia kene.
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HATU CAS:148893-10-1 Tau Faumea
HATU (1- [bis (dimethylamino) methylene] -1H-1,2,3-triazolo [4,5-b] pyridinium 3-oxid hexafluorophosphate) o se faʻaoga faʻapipiʻi masani e faʻaaogaina i le peptide synthesis ma le kemisi kemisi.
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D-fucose CAS:3615-37-0 Tau Faumea
O le D-fucose o se monosaccharide, aemaise o le ono-carbon suka, e aofia i le vaega o suka faigofie e taʻua o le hexoses.O se isomer o le kulukose, ese i le faʻatulagaina o le tasi vaega hydroxyl.
D-fucose e masani ona maua i meaola eseese, e aofia ai siama, sigi, laau, ma manu.E iai sona sao taua i le tele o gaioiga fa'aolaola, e pei o le fa'ailoina o le cell, fa'apipi'i o le cell, ma le glycoprotein synthesis.O se vaega o glycolipids, glycoproteins, ma proteoglycans, lea e aofia ai i le cell-to-cell fesootaiga ma le iloa.
I totonu o tagata, D-fucose o loʻo aʻafia foi i le biosynthesis o fausaga glycan taua, e pei o le Lewis antigens ma le vaega toto antigens, lea e iai aʻafiaga i le fesoʻotaʻiga toto ma faʻamaʻi faʻamaʻi.
D-fucose e mafai ona maua mai i punaoa eseese, e aofia ai limu, laau, ma microbial fermentation.E faʻaaogaina i suʻesuʻega ma faʻaoga faʻapitoa, faʻapea foʻi ma le gaosiga o nisi vailaʻau ma vailaʻau faʻapitoa.
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DDT CAS:3483-12-3 Tau Faumea
DL-Dithiothreitol, e lauiloa foi o le DTT, o se sui faʻaitiitia e masani ona faʻaaogaina i suʻesuʻega o meaola faʻaola ma molecular biology.O se mole la'ititi ma se vaega thiol (o lo'o i ai le sulfur) i pito ta'itasi.
O le DTT e masani ona faʻaaogaina e motusia ai fusi o le disulfide i polotini, lea e fesoasoani e faʻaalia pe faʻaumatia.O lenei faʻaitiitiga o faʻamaufaʻailoga disulfide e taua tele i suʻesuʻega suʻesuʻe eseese e pei o le faʻamamaina o porotini, gel electrophoresis, ma suʻesuʻega faʻatulagaina o porotini.E mafai fo'i ona fa'aoga le DTT e puipuia ai vaega thiol ma puipuia ai fa'ama'i i taimi o fa'ata'ita'iga.
O le DTT e masani ona fa'aopoopo i fofo fa'ata'ita'i i ni fa'atonuga laiti, ma o lona gaioiga e fa'alagolago i le i ai o le okesene.E taua tele le taulimaina ma le faʻaeteete o le DTT aua e maʻaleʻale i le ea, vevela, ma le susu, lea e mafai ona faʻaitiitia ai lona aoga.
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D-(+)-Galactose CAS:59-23-4 Tau Faumea
D-(+)-Galactose o se suka monosaccharide ma o se vaega taua o le tele o gaioiga olaola.O se suka fa'anatura e maua i le tele o mea'ai, e pei o fualaau 'aina, mea'ai susu, ma fualaau faisua.
O le galactose e masani ona fa'aosoina i totonu o le tino e ala i se fa'asologa o gaioiga fa'aenzymatic.E iai sona sao taua i fesoʻotaʻiga sela, gaosiga o le malosi, ma le biosynthesis o mole mole taua e pei o glycolipids, glycoproteins, ma le lactose.
I le tulaga o ana talosaga, D-(+)-Galactose e masani ona faʻaaogaina i microbiology ma biotechnology e avea o se puna kaponi i faʻasalalauga aganuu mo le tuputupu aʻe o microorganisms eseese.E fa'aaogaina fo'i i le gaosiga o vaila'au fa'aola, vaila'au, ma mea'ai.E le gata i lea, e masani ona faʻaaogaina e avea o se fomaʻi faʻamaʻi faʻamaʻi, aemaise i suʻega mo le suʻesuʻeina o le ate ma le suʻeina o faʻafitauli faʻapitoa e fesoʻotaʻi ma le galactose metabolism.
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beta-D-Galactose pentaacetate CAS:4163-60-4
Beta-D-Galactose pentaacetate o se vailaʻau vailaʻau e maua mai le galactose, o le suka monosaccharide.E faia i le acetylating vaega hydroxyl taitasi o le galactose mole ma vaega acetyl e lima.
O lenei tuufaatasiga e masani ona faʻaaogaina e avea o se mea e puipuia ai le galactose i gaioiga faʻamaʻi eseese ma faiga faʻapipiʻi.O le pentaacetate fomu e fesoasoani e faʻamautu le galactose ma puipuia ai faʻalavelave le manaʻomia poʻo suiga i taimi o gaioiga.
E le gata i lea, o lenei tuufaatasiga e mafai ona faʻaaogaina e fai ma mea muamua mo le tuʻufaʻatasia o isi galactose derivatives.O vaega o le acetyl e mafai ona filifilia filifilia e maua mai ai galactose eseese faʻatasi ai ma vaega faʻapitoa.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium masima CAS:129541-41-9
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt o se vailaʻau faʻapitoa e masani ona faʻaaogaina i suʻesuʻega suʻesuʻe ma suʻesuʻega.E masani ona taʻua o le X-Gluc ma e masani ona faʻaaogaina e fai ma sui mo le suʻeina o le beta-glucuronidase enzyme activity.
A iai le beta-glucuronidase, e faʻamavaeina le fusi o le glucuronide i le X-Gluc, e mafua ai le faʻasaʻolotoina o se lanu lanumoana e taʻua o le 5-bromo-4-chloro-3-indolyl.Ole tali lea e masani ona fa'aoga ile va'aia po'o le spectrophotometrically fa'aalia le fa'aaliga ole beta-glucuronidase enzyme i sela po'o masini.
Ole masima masima ole X-Gluc e faʻaleleia ai lona soluble i vaifofo vai, faʻafaigofie lona faʻaogaina i suʻega suʻesuʻe.O le X-Gluc e masani ona faʻaaogaina i suʻesuʻega o meaola mole mole e suʻesuʻe ai faʻamatalaga o kenera, gaioiga faʻalauiloa, ma suʻesuʻega a le tusitala.E mafai fo'i ona fa'aoga e iloa ai le iai o meaola e gaosia ai le beta-glucuronidase, e pei o nisi siama, i su'esu'ega microbiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9
4-Nitrophenyl-beta-D-xylopyranoside ose chromogenic substrate faʻaaogaina i suʻega enzymatic e iloa ma fuaina le gaioiga o enzymes e taʻua o beta-xylosidases.
