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MOPS sodium masima CAS:71119-22-7
MOPS sodium masima, e lauiloa foi o le 3-(N-morpholino) propanesulfonic acid sodium salt, o se mea e masani ona faʻaaogaina faʻapipiʻi i suʻesuʻega biochemical ma molecular biology.E fa'aaogaina e fa'amautu ai se tulaga fa'amautu o le pH ma fa'atupu tulaga lelei mo fa'agaioiga enzymatic, fa'amautu polotini, ma le tuputupu a'e o le aganu'u.MOPS sodium masima e sili ona aoga i le tuʻuina atu o le malosi faʻafefe i le pH vaʻaia e tusa ma le 6.5 i le 7.9.E fa'aaogaina lautele i faiga fa'amama polotini, gel electrophoresis, su'esu'ega enzyme, ma su'esu'ega aganu'u.
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D-Glucuronic acid CAS:6556-12-3
O le D-Glucuronic acid o se suka suka e maua mai le kulukose, ma e masani ona maua i totonu o le tino o le tagata ma aano eseese o laau ma manu.E iai sona sao taua i le fa'amama, fusifusia ma fa'ate'a'ese ai mea oona ma vaila'au mai le tino.E le gata i lea, o le D-Glucuronic acid o loʻo aʻafia i le tuʻufaʻatasia ma le metabolism o mole mole eseese, e aofia ai glycosaminoglycans, lea e taua mo masini fesoʻotaʻi.O lo'o i ai mea fa'aantioxidant ma fa'amanuiaga fa'alesoifua maloloina, ma e fa'aaogaina i mea'ai fa'aopoopo ma mea tau pa'u.
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2-Chloroethanesulfonic acid CAS:15484-44-3
2-Chloroethanesulfonic acid, e ta'ua fo'i ole chloroethanesulfonic acid po'o le CES, o se fa'alapotopotoga fa'alenatura ma le fua fa'a-chemical C2H5ClSO3H.O se vai manino, e leai ni lanu e sili ona solu i vai ma so'o fa'alaisa polar.
O lo'o fa'aaogaina lautele le CES e avea o se vaila'au fa'atekonolosi fa'apitoa i pisinisi eseese.O lo'o fa'aaoga muamua i le tu'ufa'atasiga o vaila'au, vaila'au fa'ato'aga, ma vali fa'aletino.O lona vaega o le sulfonic acid ua avea ai ma se fa'aoga aoga mo le fa'aofiina o le sulfonic acid functionality i totonu o meaola mole, lea e mafai ona faʻaleleia ai lo latou solubility, mautu, poʻo le bioactivity.
Ona o lona malosi malosi, e mafai foʻi ona faʻaaogaina le CES e fai ma faʻamaʻi poʻo se faʻamaʻi faʻamaʻi i gaioiga faʻalaʻau.O lona natura oona e mafai ai ona fa'aolaina gaioiga e pei o esterifications, acylations, ma sulfonations.E le gata i lea, e mafai ona avea o se pH adjuster, buffering agent, poʻo le inhibitor corrosion i faiga faʻapisinisi.
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PIPES monosodium masima CAS:10010-67-0
Sodium hydrogen piperazine-1,4-diethanesulphonate, e taʻua foi o le HEPES-Na, o se faʻaoga faʻapipiʻi masani i suʻesuʻega o meaola ma meaola.E fesoasoani e fa'atumauina le tulaga mautu o le pH o le 6.8 i le 8.2 i fa'aoga eseese, e aofia ai le aganu'u cell, su'ega enzyme, ma metotia mole biology.HEPES-Na e fetaui ma faiga fa'aola eseese ma e mautu i luga o le lautele o le vevela.
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4-Aminophenyl-β-D-galactopyranoside CAS:5094-33-7
4-Aminophenyl-β-D-galactopyranoside o se vailaʻau faʻapipiʻi e tutusa ma le substrate 3-Nitrophenyl-β-D-galactopyranoside (ONPG).E faʻaaogaina e fai ma sui mo suʻega o le enzyme beta-galactosidase. A faʻafefeteina le 4-aminophenyl-β-D-galactopyranoside e le beta-galactosidase, e faʻasaʻo ai se mea lanu samasama e taʻua o le p-aminophenol.O le gaioiga o le beta-galactosidase e mafai ona fuaina e ala i le faʻatusatusaina o le aofaʻi o le p-aminophenol e gaosia, e masani lava e ala i se colorimetric poʻo le spectrophotometric assay.O lenei substrate e masani ona faʻaogaina faʻatasi ma isi mea faʻapitoa ma analogs o le lactose e suʻesuʻe ai le gaioiga beta-galactosidase, faʻamatalaga o gene. , enzyme inhibition po'o le fa'agaoioia, ma fa'amaonia siama.O le mafai ona iloa ma fua le gaioiga beta-galactosidase e taua tele i le tele o vaega o suʻesuʻega, e aofia ai mole mole biology, microbiology, ma suʻesuʻega falemaʻi.
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3-(cyclohexylamino)-2-hydroxy-1-propanesuhicic acid CAS:73463-39-5
3-(cyclohexylamino) -2-hydroxy-1-propanesuhicic acid o se vailaʻau faʻamaʻi ma le fua mole C12H23NO3S.E a'afia i se aiga o mea fa'afefiloi e ta'ua o sulfonic acids.O lenei fa'apotopotoga fa'apitoa o lo'o i ai se vaega o le cyclohexylamino, o le hydroxy group, ma le propanesuhicic acid moiety.E fa'aaogaina i fa'alapotopotoga fa'apisinisi eseese, e aofia ai o se poloka faufale i le fa'aogaina o meaola ma le fa'aogaina i su'esu'ega fa'afoma'i.O le fausaga tulaga ese o le tuufaatasiga ma meatotino ua talafeagai ai mo gaioiga faapitoa o vailaau ma suesuega faasaienisi.
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HEIDA CAS:93-62-9 Tau Faumea
N-(2-Hydroxyethyl)iminodiacetic acid (HEIDA) o se vailaʻau vailaʻau ma le tele o faʻaoga i vaega eseese.O se sui chelating, o lona uiga o loʻo i ai le malosi e fusifusia i ions uʻamea ma fausia ai faʻalavelave faʻamautu.
I le kemisi su'esu'e, e masani ona fa'aogaina le HEIDA e fai ma sui lavelave i titrations ma va'aiga au'ili'ili.E mafai ona fa'aaogaina e fa'asolo ai iona u'amea, e pei o le calcium, magnesium, ma le u'amea, ma taofia ai i latou mai le fa'alavelave i le sa'o o fua fa'atatau.
E maua fo'i e le HEIDA le fa'aogaina i totonu o fale talavai, aemaise i le fa'atulagaina o nisi vaila'au.E mafai ona faʻaaogaina e avea o se faʻamautu ma solubilizing solu mo vailaʻau e le mafai ona soluble, fesoasoani e faʻaleleia lo latou bioavailability ma le aoga.
O le isi vaega o le fa'aoga mo HEIDA o lo'o i totonu o le fanua o vai otaota ma le fa'aleleia o le siosiomaga.E mafai ona fa'afaigaluegaina e avea o se sui fa'amama e aveese ai le u'amea mamafa mai le vai po'o le palapala, ma fa'aitiitia ai lo latou o'ona ma fa'aolaina ai taumafaiga toe fa'aleleia.
E le gata i lea, o le HEIDA ua faʻaaogaina i le tuʻufaʻatasia o faʻamaopoopo faʻamaopoopo ma faʻavae uʻamea-organic (MOFs), o loʻo i ai faʻaoga eseese i le catalysis, teuina o kesi, ma le lagona.
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2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2
2-Nitrophenyl-beta-D-glucopyranoside o se vailaʻau vailaʻau e aofia ai se mole mole glucopyranoside faʻapipiʻi i se vaega nitrophenyl.E masani ona faʻaaogaina e fai ma sui i suʻega enzymatic e iloa ma faʻatusatusa le gaioiga o enzymes pei ole beta-glucosidase.O le vaega o le nitrophenyl e mafai ona vavaeina e le enzyme, e mafua ai le tuʻuina atu o se oloa lanu samasama e mafai ona fuaina spectrophotometrically.O lenei tuufaatasiga e sili ona aoga i le suʻesuʻeina o enzyme kinetics ma suʻesuʻega maualuga o le enzyme inhibitors poʻo le activators.O lo'o fa'afaigaluegaina fo'i i su'esu'ega fa'aolaola mo le su'esu'eina o le metabolism o le ga'o ma e pei o se mea e fa'atatau i le glycosidic-linkage-specific substrate.
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MES HEMISODIUM SALT CAS:117961-21-4
2-Amino-2-methyl-1,3-propanediol, e taʻua foi o le AMPD poʻo le α-methyl serinol, o se vailaʻau vailaʻau faʻatasi ma le faʻasologa mole C4H11NO2.O se amino ava malosi e masani ona faʻaaogaina e avea o se vailaʻau vavalo i le tuʻufaʻatasiga o vailaʻau ma vailaʻau faʻalaʻau.AMPD ua lauiloa mo lona gafatia e galue o se fesoasoani chiral i asymmetric tali, ma taua i le gaosiga o enantiomerically mama tuufaatasiga.E le gata i lea, ua faʻaaogaina e fai ma mea e faʻaogaina i le tausiga o le tagata lava ia ma mea faʻalelei mo ona mea faʻasusu.
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Tris(hydroxymethyl)nitromethane CAS:126-11-4
Tris (hydroxymethyl) nitromethane, e masani ona taʻua o Tris poʻo THN, o se vailaʻau faʻamaʻi ma le fua mole C4H11NO4.Ose ma'a tioata samasama samasama e sili ona so'u ile vai.Tris o loʻo faʻaaogaina lautele e fai ma sui faʻapipiʻi i faʻaoga o meaola faʻapitoa ma molecular biology.E fesoasoani i le faatumauina o se tulaga mautu o le pH i vaifofo, e taua tele mo auala eseese e pei ole DNA ma le RNA vavae ese, PCR, gel electrophoresis, faʻamamaina o porotini, aganuʻu sela, kemisi porotini, enzymology, ma suʻesuʻega o meaola.O mea fa'apolopolo a Tris e mafai ai ona maua tulaga sili ona lelei i nei su'esu'ega, fa'amautinoa i'uga sa'o ma fa'atuatuaina.
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FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9
Fluorescein mono-beta-D-galactopyranoside, lea e taʻua foʻi o le FMG, o se mea faʻapipiʻi felafolafoaʻi e masani ona faʻaaogaina e fai ma sui i suʻesuʻega o meaola faʻaola ma cell biology.E maua mai i le methyl-beta-D-galactopyranoside e ala i le tuʻufaʻatasia ma se mole o le fluorescein. E masani ona faʻaaogaina le FMG e suʻesuʻe ai le gaioiga o le beta-galactosidase, o se enzyme e faʻamalosia ai le hydrolysis o le lactose i le galactose ma le kulukose.I le faʻaaogaina o le FMG e fai ma sui, e mafai e tagata suʻesuʻe ona mataʻituina le gaioiga o le enzymatic o le beta-galactosidase e ala i le fuaina o le faʻafefe o le fluorescence.O le hydrolysis o le FMG e le beta-galactosidase e taʻitaʻia ai le tuʻuina atu o le fluorescein, e mafua ai le faʻateleina o le faʻailoga o le fluorescent e mafai ona faʻatusatusaina.O lenei faʻapipiʻi e faʻaaogaina foi e suʻesuʻe ai le iloa o le carbohydrate ma fegalegaleaiga.E mafai ona fa'aogaina le FMG e fai ma su'esu'ega mole mole e su'esu'e ai le so'otaga o lectins (porotini e pipii fa'apitoa i ga'o) i galactose e iai ga'o.O le fusifusia o le FMG-lectin complexes e mafai ona iloa ma faʻatatauina e faʻavae i luga o suiga o le fluorescence emission. I le aotelega, o le FMG o se meafaigaluega faʻapitoa i le suʻesuʻeina o gaioiga enzyme ma le faʻalauiloaina o le carbohydrate, e ofoina atu ai se auala faigofie ma maaleale e fua ai le fluorescence ma iloilo nei gaioiga faʻasolosolo.
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3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7
3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPANAL, e ta'ua fo'i o le NBD-aldehyde, ose fa'aputuga masani ona fa'aogaina i su'esu'ega o mea olaola ma molecular biology.
