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2,3,4,6-TETRA-O-BENZOYL-ALPHA-D-GLUCOPYRANOSYL BROMIDE CAS:14218-11-2
2,3,4,6-Tetra-O-benzoyl-alpha-D-glucopyranosyl bromide o se vailaʻau vailaʻau e aʻafia i le vasega o suka.E aofia ai se mole mole kulukose ma vaega e fa benzoyl o loʻo faʻapipiʻi i ona vaega hydroxyl, faʻatasi ai ma le bromide atom i le tulaga anomeric.
O lenei tuufaatasiga e masani ona faʻaaogaina i kemisi faʻalaʻau ma vailaʻau e avea o se vaega puipuia mo le gaioiga o le hydroxyl o le kulukose.O vaega o le benzoyl o lo'o fa'aogaina e pupuni mo sina taimi le vaega o le hydroxyl reactive, ma fa'aitiitia ai le a'afia i fa'a'aga o vaila'au e le'o mana'omia a'o fa'agasolo fa'agaioiga.Ole mea lea e mafai ai ona fa'atinoina filifiliga ole vaega ole hydroxyl fa'apitoa ile kulukose.
E le gata i lea, e mafai ona faʻaaogaina le kulukose puipuia o le benzoyl e fai ma poloka fale mo le faʻapipiʻiina o glycosides ma glycoconjugates eseese.Glycosides o mea faʻapipiʻi e fausia i le soʻotaga o se mole suka i se isi vaega, e pei o se vailaʻau poʻo se mea faʻanatura, ma latou maua ai faʻaoga i le atinaʻeina o vailaʻau ma le biology kemisi.
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POPSO CAS:68189-43-5 Tau Faumea
POPSO, puupuu mo Piperazine-N, N'-bis (2-hydroxypropanesulfonic acid) sesquisodium masima, o se sui faʻapipiʻi e masani ona faʻaaogaina i suʻesuʻega o meaola ma meaola.E fesoasoani e fa'amautu le maualuga o le pH i vaifofo, aemaise lava ile vaega ole pH ole tino.PIPES sesquisodium masima o loʻo faʻaaogaina i le aganuʻu, protein biochemistry, electrophoresis, molecular biology techniques, faʻaogaina o vailaʻau, ma isi mea.O lona gafatia e faʻatonutonu le pH e avea ai ma mea faigaluega taua i suʻesuʻega eseese ma faʻalapotopotoga faʻapisinisi.
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BES CAS:10191-18-1 Tau Faumea
N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid, e taʻua foi o le BES poʻo le N, N-Bis (2-hydroxyethyl) aminoethanesulfonic acid, o se vailaʻau vailaʻau e masani ona faʻaaogaina e fai ma sui faʻafefe i le tele o faʻasaienisi ma alamanuia talosaga. .
O le BES o le zwitterionic compound, o lona uiga e iai uma moliaga lelei ma leaga i totonu o lona fausaga.O lenei meatotino e mafai ai ona tausia lelei se pH mautu i fofo.
BES o loʻo i ai le tau o le pKa e tusa ma le 7.4, e faʻapitoa ai le aoga mo le paʻu i tulaga pH o le tino.E masani ona faʻaaogaina i suʻesuʻega o meaola ma meaola faʻapitoa, e pei o le faʻamamaina o porotini, enzyme reactions, ma le aganuʻu sela, lea e taua tele ai le tausisia o se pH patino.
E le gata i lea, o le BES o loʻo faʻaaogaina lautele i le electrophoresis techniques, aua e fesoasoani e faʻamautu le pH e manaʻomia mo le vavaeeseina ma le auʻiliʻiliga o biomolecules molia, e pei o polotini ma nucleic acids.
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2,3,4,6-Tetra-O-acetyl-α-D-galactopyranosyl 2,2,2-trichloroacetimidate CAS:86520-63-0
2,3,4,6-Tetra-O-acetyl-α-D-galactopyranosyl 2,2,2-trichloroacetimidate o se vailaʻau vailaʻau e masani ona faʻaaogaina i kemisi carbohydrate ma glycosylation reactions.O se mea e maua mai i le α-D-galactopyranose, o se ituaiga o suka, lea o loʻo faʻapipiʻiina ai vaega o le hydroxyl i le 2, 3, 4, ma le 6 o le galactopyranose mama.E le gata i lea, o le anomeric carbon (C1) o le suka e puipuia i se vaega trichloroacetimidate, lea e avea ai ma se eletise malosi i taimi o gaioiga glycosylation.
E masani ona faʻaaogaina le faʻapipiʻi e fai ma sui o le glycosylating e faʻafeiloaʻi ai le galactose moieties i mole mole eseese, e pei o polotini, peptides, poʻo ni meaola laiti.E mafai ona ausia lenei mea e ala i le fa'afo'iina o lenei mea fa'atasi ma se nucleophile (fa'ata'ita'iga, vaega hydroxyl i luga o le mole mole fa'atatau) i lalo o tulaga talafeagai.O le vaega trichloroacetimidate e faafaigofieina le faapipiiina o le galactose moiety i le mole taulaʻiga, e mafua ai le fausia o se fusi glycosidic.
O lenei tuufaatasiga e masani ona faʻaaogaina i le faʻaogaina o glycoconjugates, glycopeptides, ma glycolipids.O lo'o ofoina mai ai se auala fa'apitoa ma lelei mo le fa'aleleia o molela'au ma toega galactose, lea e mafai ona fa'atatau i vaega eseese, e aofia ai su'esu'ega fa'aolaola, faiga tu'uina atu vaila'au, po'o le atina'eina o tui.
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3-(N,N-dimethyldodecylammonio) propanesulfonate CAS:14933-08-5
N-(2-Aminoethyl)morpholine, e taʻua foi o le AEM, o se vailaʻau vailaʻau ma se fausaga laina.E aofia ai se mama morpholine ma se vaega aminoethyl o loʻo faʻapipiʻi i se tasi o ona atoms nitrogen.O le AEM o se vai e leai ni lanu ma se manogi manogi.
E maua e le AEM le fa'aogaina i vaega eseese o alamanuia.E masani ona faʻaaogaina e avea o se solvent mo faʻalapotopotoga faʻapitoa ona o lona tulaga sili ona lelei.E le gata i lea, o le AEM o loʻo galue o se faʻalavelave faʻaleagaina i alamanuia e aofia ai le faʻamamaina o uʻamea, gaosiga o le suauʻu ma le kesi, ma togafitiga vai.E fesoasoani e puipuia u'amea mai 'ele ma pala.
E le gata i lea, o le AEM e avea o se vailaʻau vavalo mo le tuʻufaʻatasia o vailaʻau, vailaʻau faʻatoʻaga, ma vailaʻau faʻapitoa.O loʻo faʻaaogaina i mea faʻapipiʻi polymer e faʻaleleia ai mea faʻapipiʻi o paʻu, faʻapipiʻi, ma faʻamau.O lo'o fa'aaogaina fo'i le AEM e fai ma fa'anofoaa le pH po'o se sui fa'apolopolo i nisi o faiga fa'apisinisi.
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MES monohydrate CAS:145224-94-8
O le MES monohydrate o le fa'amama vai o le 4-Morpholineethanesulfonic acid (MES), o se mea fa'apipi'i e masani ona fa'aaogaina i su'esu'ega fa'ale-ola ma mea fa'aola.Ose pa'epa'e tioata pa'epa'e e solu ile vai ma e iai lona tau pKa ile 6.1.MES monohydrate ua lauiloa ona o lona gafatia e faatumauina se pH mautu i le va o le 5.5 i le 6.7, e talafeagai mo faʻaoga eseese e pei o suʻesuʻega enzyme, faʻamamaina o le protein, gel electrophoresis, aganuʻu cell, ma vailaʻau faʻamaʻi.O lona fegalegaleai ma le fetaui lelei ma faiga fa'aola ua avea ai ma vaega taua i le tele o su'esu'ega ma fa'ata'ita'iga.
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beta-d-glucose pentaacetate CAS:604-69-3
Beta-D-glucose pentaacetate o se vailaʻau faʻamaʻi e maua mai le kulukose, o se suka faigofie.E fausia e ala i le acetylating kulukose ma vaega acetyl e lima, e mafua ai le faʻapipiʻiina o nei vaega i le hydroxyl (OH) vaega o loʻo i totonu o le kulukose mole.O lenei suiga ole kulukose e maua ai le fa'aleleia atili o le mautu ma mafai ai ona solu i so'o so'o.
O le Beta-D-glucose pentaacetate o loʻo i ai faʻaoga eseese i le matata o kemisi faʻalaʻau, aemaise i le faʻapipiʻiina ma le fesuiaiga o gaʻo.E mafai ona avea o se mea muamua poʻo se vaeluagalemu i le sauniuniga o isi mea e maua mai ai le carbohydrate poʻo mea faʻapipiʻi lavelave.E le gata i lea, ua faʻaaogaina i nisi o vailaʻau faʻafomaʻi ma vailaʻau e pei o le tuʻuina atu o vailaʻau ma faʻatonuga faʻasaʻo.
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Tris-HCl CAS:1185-53-1 Tau Faumea
Tris-HCl, e taʻua foi o Tris hydrochloride, o se faʻaoga masani o meaola faʻaoga i faʻaoga eseese.O se tuufaatasiga o le Tris (tris(hydroxymethyl)aminomethane) ma le hydrochloric acid.O lenei faiga faʻapipiʻi e aoga i le faatumauina o se siosiomaga faʻamautu pH, aemaise ile va ole pH 7-9.Tris-HCl o loʻo faʻaaogaina lautele i metotia mole mole biology, protein biochemistry, enzymology, ma isi mea faʻaoga faʻapitoa.E fesoasoani i le faatumauina o tulaga sili ona lelei o le pH e manaʻomia mo nei faiga, faʻamautinoa le mautu ma le gaioiga o polotini, enzymes, ma nucleic acids.O lo'o maua le Tris-HCl i ituaiga eseese, e pei o le pauta po'o vaifofo fa'ato'a, e fa'afaigofie ai ona saunia ma fa'aoga i nofoaga eseese falesu'esu'e.
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N-ethyl-N-(3-sulfopropyl)-M-anisidinesodium CAS:82611-88-9
N-Ethyl-N-(3-sulfopropyl) -3-methoxyaniline sodium salt o se vailaʻau vailaʻau o loʻo i ai se vaega N-ethyl, se vaega sulfopropyl, ma se vaega 3-methoxyaniline.E masani ona i ai o se masima sodium, lea e faʻaleleia ai lona soluble i le vai.
O lenei fa'aputuga o lo'o fa'aoga eseese i tulaga tau alamanuia ma su'esu'ega.E mafai ona fa'aogaina e fai ma vali va'ava'ai, fa'amamafa, po'o se reagent i le fa'aogaina o meaola.O ona meatotino, e pei o le solubility, mautu, ma le gaoioi, e talafeagai mo faʻaoga faʻapitoa i vaega eseese.
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2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE CAS:312693-81-5
2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE o se vailaʻau vailaʻau e masani ona faʻaaogaina i suʻesuʻega ma suʻesuʻega o meaola.O se mea e maua mai i le galactose, o se ituaiga suka.E masani ona faʻaaogaina le faʻapipiʻi e fai ma mea e suʻe ai le gaioiga o le beta-galactosidase, o se enzyme o loʻo i ai i le tele o meaola, e aofia ai siama. Pe a iai le beta-galactosidase, e pipii 2-NAPHTHYL-BETA-D-GALACTOPYRANOSIDE i le naphthol ma le galactose.O le naphthol molecule e mafai ona faigofie ona iloa e ala i lona faʻaaogaina o le malamalama ultraviolet, e mafai ai e saienitisi ona fua le gaioiga o le beta-galactosidase.O lenei su'esu'ega e masani ona fa'aaogaina i su'esu'ega mole mole ma genetics, mo fa'aoga e pei o le su'esu'eina o le fa'atonutonuina o kenera, fa'amatalaga porotini, ma le fa'aogaina o le sela.
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TOOS CAS:82692-93-1 Tau Faumea
Sodium 3-(N-ethyl-3-methylanilino) -2-hydroxypropanesulfonate o se vailaʻau faʻamaʻi e masani ona taʻua o MESNa.E fa'aaoga muamua e fai ma sui fa'aitiitiga i le biochemistry ma le biology mole.MESNa o loʻo i ai le malosi e talepe ai fusi disulfide i polotini, faʻaliliuina i latou i vaega sulfhydryl.O lenei faiga fa'aitiitiga e mana'omia mo fa'aoga eseese e pei o le denaturation o porotini, puipuia le fa'aputuina o polotini, fa'ailogaina o polotini, ma le toe fo'i o le polotini.MESNa e taua tele i le faʻaogaina o polotini, auʻiliʻiliga, ma suiga i suʻesuʻega faasaienisi.
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CAPSO Na CAS:102601-34-3 Tau Faumea
CAPSO Na, e lauiloa foi o le 3-(cyclohexylamino) -2-hydroxy-1-propanesulfonic acid sodium salt, o se tuufaatasiga e patino i le aiga o sulfonic acids.Ose zwitterionic buffer e masani ona fa'aogaina i fa'aoga fa'aolaola ma mole mole.
O le CAPSO Na e galue e avea o se vailaʻau faʻatonutonu pH ma e faʻaaogaina lautele i faʻapipiʻi paʻu e faʻamautu ai se pH mautu i se vaega patino.E iai lona tau pKa e tusa ma le 9.8 ma e masani ona faʻaaogaina i suʻega e manaʻomia ai le pH i le va o le 8.5 ma le 10.
O le masima masima o le CAPSO (CAPSO Na) e faʻaleleia ai le solubility ma le faigofie o le taulimaina pe a faʻatusatusa i le fua ole acid form.E solu vai ma faigofie ona fai ni vaifofo mautu i tulaga eseese, e fa'afaigofie ai mo su'esu'ega eseese.
O nisi o fa'aoga masani a le CAPSO Na e aofia ai le avea o se pa'u i faiga fa'a eletise, su'esu'ega o le enzyme, fa'amamāina o porotini, ma fa'asalalauga tu'ufa'atasiga.O lona malosi e fa'apalepale ai ma le fetaui lelei ma faiga fa'aola e fesoasoani i lona aoga i nei matā'upu.
