ONPG CAS:369-07-3 Tau Faumea

O le aʻafiaga o le ONPG e avea o se mea faʻapipiʻi e tatau ona vavaeina e le enzyme β-galactosidase, e mafua ai le tuʻuina atu o se oloa samasama, o-nitrophenol.O lenei suiga lanu e mafai ona fuaina spectrophotometrically, e mafai ai mo le quantification o le β-galactosidase gaoioiga.O le faʻaaogaina o le ONPG o loʻo faʻamuamua i le iloiloga o faʻamatalaga o le kenera i le biology mole ma suʻesuʻega microbiology.E masani ona faʻaaogaina e fua ai le gaioiga o le β-galactosidase e avea o se tusitala mo suʻesuʻega faʻamatalaga o kene, aemaise i siama e pei ole E. coli.O le lacZ gene, lea e faʻaogaina ai le β-galactosidase, e masani ona faʻaaogaina e fai ma faʻailoga mo le suʻesuʻeina o faʻamatalaga o le kenera, ona o lona faʻaaliga e mafai ona faʻaosoina e tulaga faʻapitoa poʻo le pulea e faʻasalalauga faʻapitoa. fa'aaliga o kenera e ala i le fuaina o le gaioiga o le β-galactosidase.O lenei suʻega e faʻaaogaina lautele i faʻaoga eseese, e pei o le suʻesuʻeina o le faʻalauiloaina o gaioiga, faʻatonuga o gene, ma fegalegaleaiga protein-protein.E le gata i lea, e mafai ona faʻaaogaina e fuafua ai kinetics enzyme ma iloilo aʻafiaga o suiga poʻo togafitiga ile gaioiga ole enzyme.