P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Su'esu'ega ole gaioioiga beta-galactosidase: O le PNPG e masani ona fa'aogaina i su'ega e fua ai le gaioiga ole beta-galactosidase, ose enzyme e fa'aosoina ai le fa'asusu o le lactose ile kulukose ma le galactose.O le hydrolysis o le PNPG e le beta-galactosidase e faʻasaʻo ai se mole mole p-nitrophenol (pNP), lea e mafai ona iloa spectrophotometrically ona o lona lanu samasama.

Suʻesuʻega mo faʻamaʻi faʻamaʻi ma faʻagaioiga: E mafai ona faʻaogaina le PNPG i suʻesuʻega maualuga e iloa ai mea faʻapipiʻi e faʻaogaina le gaioiga beta-galactosidase.E ala i le fuaina o le fua faatatau o le PNPG hydrolysis i le i ai o suʻega suʻega eseese, e mafai e tagata suʻesuʻe ona faʻamaonia inhibitors e faʻaitiitia ai le gaioiga o le enzyme poʻo le faʻamalosi e faʻaleleia ai le gaioiga o le enzyme.

Suesuega o enzyme kinetics: O le hydrolysis o le PNPG e beta-galactosidase e mulimuli i Michaelis-Menten kinetics, e mafai ai e tagata suʻesuʻe suʻesuʻe ona fuafua mea taua ole enzyme e pei ole maualuga ole saoasaoa ole tali (Vmax) ma le Michaelis tumau (Km).O nei fa'amatalaga e fesoasoani i le malamalama i le fa'aogaina o le substrate o le enzyme ma le fa'aogaina o le catalytic.

Talosaga mole mole biology: Beta-galactosidase, lea e tipi ai le PNPG, e masani ona faʻaaogaina e fai ma faʻamatalaga faʻamatalaga i le biology mole.O le PNPG substrate e masani ona faʻaaogaina e iloa ai ma vaʻaia le faʻaaliga o le kenera fai lipoti, e maua ai se auala faigofie ma maaleale e iloilo ai faʻamatalaga o kenera i faiga faʻataʻitaʻiga eseese.