Phenylgalactoside CAS:2818-58-8

A'afiaga ile gaioiga ole enzyme: E masani ona fa'aogaina le Phenylgalactoside e fua ai le gaioiga ole enzyme β-galactosidase.A fa'asusu le phenylgalactoside e le β-galactosidase, na te fa'asa'olotoina p-nitrophenol.O le faʻaputuina o le p-nitrophenol e mafai ona fuaina faʻatatau, e maua ai faʻamatalaga i le gaioiga o le β-galactosidase.O lenei aafiaga e fa'aogaina i fa'aoga e pei ole su'ega ole enzyme ma faiga su'esu'e.

Su'esu'ega o fa'amatalaga o le kenera: E masani ona fa'aogaina le Phenylgalactoside e fai ma sui i su'esu'ega o meaola mole mole e su'esu'e ai fa'amatalaga o kenera.O le lacZ gene, lea e fa'ailoga β-galactosidase, e masani ona fa'afefiloi ma fa'asologa fa'atonutonu o isi kenera e fiafia i ai.O le fa'aaliga o le lacZ gene ma le hydrolysis o le phenylgalactoside e le β-galactosidase e mafai ona fa'aalia ai le fa'aaliga fa'atusa ma le maualuga o le ga'o fa'atatau o lo'o su'esu'eina.

Faiga su'esu'e: Phenylgalactoside o se vaega taua o faiga su'esu'e e fa'aogaina ai le gaioiga β-galactosidase.O se tasi o fa'ata'ita'iga lauiloa o le su'esu'ega lanumoana-pa'epa'e, lea e fa'aaogaina e iloa ai sela toe tu'ufa'atasia pe suia i su'esu'ega o meaola mole mole.O kolone na fa'auluina lelei le DNA recombinant po'o le toe fa'atupuina o le kenera o le a fa'aalia le β-galactosidase, e o'o atu ai i le hydrolysis o le phenylgalactoside ma le fa'avaeina o se lanu lanumoana.

Fa'amamāina o polotini: I nisi tulaga, e mafai ona fa'aogaina le phenylgalactoside e fai ma ligand mo chromatography affinity e fa'amamā ai polotini e pipii fa'apitoa pe fa'agaoioia e le β-galactosidase.O le polotini o tului atonu e iai se pine fa'atasi po'o se fusu'aga fa'atasi o lo'o i ai le β-galactosidase-binding domain.E ala i le pasi atu o le paluga polotini i totonu o se koluma ma le phenylgalactoside immobilized, o le polotini manaʻomia e mafai ona filifili filifilia ma mulimuli ane faʻafefe.