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2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2
2-Nitrophenyl-beta-D-glucopyranoside o se vailaʻau vailaʻau e aofia ai se mole mole glucopyranoside faʻapipiʻi i se vaega nitrophenyl.E masani ona faʻaaogaina e fai ma sui i suʻega enzymatic e iloa ma faʻatusatusa le gaioiga o enzymes pei ole beta-glucosidase.O le vaega o le nitrophenyl e mafai ona vavaeina e le enzyme, e mafua ai le tuʻuina atu o se oloa lanu samasama e mafai ona fuaina spectrophotometrically.O lenei tuufaatasiga e sili ona aoga i le suʻesuʻeina o enzyme kinetics ma suʻesuʻega maualuga o le enzyme inhibitors poʻo le activators.O lo'o fa'afaigaluegaina fo'i i su'esu'ega fa'aolaola mo le su'esu'eina o le metabolism o le ga'o ma e pei o se mea e fa'atatau i le glycosidic-linkage-specific substrate.
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Tris Base CAS:77-86-1 Tau Faumea
Tris Base, e taʻua foi o Tromethamine poʻo THAM, o se faʻalapotopotoga faʻapitoa e masani ona faʻaaogaina i le matata o le biochemistry ma le biology mole.O se pa'epa'e, tioata tioata e sili ona solu i le vai ma e iai se manogi amine uiga.E masani ona faʻaaogaina le Tris Base e fai ma sui faʻapipiʻi e faʻamautu ai se pH mautu i suʻesuʻega faʻale-aganuʻu eseese ma taualumaga, e pei o suʻesuʻega DNA ma porotini.E mafai foʻi ona faʻaaogaina i le faʻatulagaina o vailaʻau faʻamaʻi ma i le gaosiga o mea faʻamalosi i luga.I le aotelega, o le Tris Base o se vaega taua i le tele o suʻesuʻega suʻesuʻe lea e taua tele le tausisia o se pH saʻo.
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4-Morpholineethanesulfonic acid CAS:4432-31-9
4-Morpholineethanesulfonic acid, e masani ona taʻua o le MES, o se zwitterionic compound lea e avea o se sui faʻapipiʻi i suʻesuʻega o meaola ma meaola.E fesoasoani e fa'amautu le pH mautu ile 6-7.5 ma e fa'aaogaina lautele ona o le maualalo o le oona ma le feso'ota'iga ma faiga fa'aola ma enzymes eseese.O lo'o fa'aaogaina le MES i le electrophoresis, su'esu'ega o le enzyme, aganu'u cell, fa'amamāina o le polotini, ma isi fa'ata'ita'iga fa'ata'ita'iga e mana'omia ai le pulea lelei o le pH.
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4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7
4-Nitrophenyl-beta-D-glucopyranoside o se mea e masani ona faʻaaogaina i suʻesuʻega biochemical e iloilo ai le gaioiga o enzymes pei ole β-glucuronidase.O lenei tuufaatasiga e faʻafefeteina e le enzyme, e mafua ai le tuʻuina atu o le 4-nitrophenol, lea e mafai ona fuaina e ala i le spectrophotometry.O lona fa'aogaina e mafai ai e tagata su'esu'e ona su'esu'e vaega eseese o le metabolism o vaila'au, toxicology, ma su'esu'ega fa'afoma'i e feso'ota'i ma glucuronidation reactions.
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MOPSO sodium masima CAS:79803-73-9
MOPSO sodium masima ose vaila'au fa'atupu mai le MOPS (3-(N-morpholino) propanesulfonic acid).O le zwitterionic buffer masima, o lona uiga o loʻo i ai uma se totogi lelei ma le leaga, lea e mafai ai ona faʻatumauina lelei le faʻamautuina o le pH i suʻesuʻega faʻapitoa ma meaola faʻapitoa.
O le masima masima o le MOPSO e ofoina atu tulaga lelei e pei o le faʻaleleia atili o le soluble i vaifofo vai, faʻafaigofie ona taulimaina ma saunia.E masani ona faʻaaogaina e fai ma sui faʻapipiʻi i le aganuʻu aganuʻu feaveaʻi, molecular biology techniques, suʻesuʻega o porotini, ma faʻaogaina o enzyme.
MOPSO sodium masima e fesoasoani i le faatumauina o le pH o le tuputupu aʻe ala ile aganuʻu, maua ai se siosiomaga mautu mo le tuputupu aʻe ma galuega.I le molecular biology techniques, e faʻamautu ai le pH o faʻafefiloi faʻafefiloi ma taʻavale paʻu, faʻamautinoa le saʻo ma le faʻatuatuaina iʻuga i le DNA ma le RNA vavae ese, PCR, ma gel electrophoresis.
E faʻaaogaina foʻi i le suʻesuʻeina o polotini, e fai ma sui faʻapolopolo i le taimi o le faʻamamaina o porotini, faʻatusatusaga, ma le electrophoresis.MOPSO sodium masima e fa'amautinoa tulaga sili ona lelei o le pH mo le mautu ma le gaioiga o porotini i nei faiga.
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4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4
4-Methylumbelliferyl-beta-D-glucopyranoside o se mea e masani ona faʻaaogaina i suʻega enzymatic e suʻesuʻe ai le gaioiga o enzymes beta-glucosidase.Pe a faʻatinoina e le beta-glucosidase, e oʻo i le hydrolysis, e mafua ai le tuʻuina atu o le 4-methylumbelliferone, lea e mafai ona iloa ma faʻatusatusa e faʻaaoga ai le spectroscopy fluorescence.O lenei tuufaatasiga o loʻo faʻaaogaina lautele i matāʻupu o le biochemistry, molecular biology, ma le biotechnology mo suʻesuʻega o le enzyme ma suʻesuʻega.O lona uiga fluorescence e sili ona maaleale ma talafeagai mo faʻaoga maualuga.
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METHYL Beta-D-GLUCOPYRANOSIDE HEMIHYDRATE CAS:7000-27-3
Methyl beta-D-glucopyranoside hemihydrate o se vailaʻau vailaʻau e aofia i le vasega o glucopyranosides.O se pauta tioata pa'epa'e e so'a i le vai.E masani ona fa'aogaina lenei fa'aputuga e fai ma puna ga'o i totonu o fa'asalalauga fa'aleaganu'u sela ma se mea e fa'aogaina ai gaioiga fa'a-enzymatic i su'esu'ega biochemical ma biotechnological.E mafai ona avea o se faʻataʻitaʻiga faʻataʻitaʻiga e suʻesuʻe ai le metabolism carbohydrate, felauaiga, ma le faʻaogaina i faiga faʻaola eseese.Methyl beta-D-glucopyranoside hemihydrate e maua ai talosaga i le tulaga o le glycobiology, enzymology, ma le atinaʻeina o vailaʻau, lea e faʻaaogaina e fai ma meafaigaluega faʻapipiʻi mo suʻega eseese ma suʻega.
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AMPSO CAS: 68399-79-1 Tau Faumea
AMPSO, poʻo le 3- [(1,1-dimethyl-2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, o se paʻu zwitterionic e masani ona faʻaaogaina i suʻesuʻega o meaola ma meaola.O loʻo i ai le tau o le pKa e tusa ma le 7.9, e fetaui lelei mo le faʻamautuina o tulaga pH mautu i le tele o faʻataʻitaʻiga faʻataʻitaʻiga.AMPSO e masani ona faʻaaogaina i le faʻasalalauga tuʻufaʻatasiga o le cell, faʻamamaina o porotini, suʻega enzyme, gels electrophoresis, ma DNA sequencing.E fesoasoani i le faatumauina o le tulaga manaomia o le pH, faʻamautinoaina tulaga sili ona lelei mo le tuputupu aʻe o sela, faʻamautu o le protein, gaioiga o le enzyme, ma le vavaeeseina saʻo ma le auʻiliʻiliga o biomolecules. Faatasi ai ma lona gafatia e tetee atu i suiga o le pH e mafua mai i le faʻaopoopoina o acids poʻo faavae, AMPSO o se meafaigaluega taua i totonu. fa'atumauina sa'o le fa'atonuga o le pH i le tele o su'esu'ega fa'aola ma kemikolo.
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Bis-tris hydrochloride CAS:124763-51-5
O le Bis-tris hydrochloride ose fa'aputuga fa'atasi ma mea fa'apolopolo e masani ona fa'aogaina i su'esu'ega fa'aolaola ma mea fa'aola.E fesoasoani i le faatumauina o se pH mautu ma e faʻaaogaina i le electrophoresis protein, suʻesuʻega o le enzyme, aganuʻu sela, ma vailaʻau.O lana galuega autu o le tetee atu lea i suiga i le pH pe a faʻaopoopo acids poʻo faʻavae i se vaifofo, ma avea o se meafaigaluega taua i le tele o faʻasaienisi ma alamanuia talosaga.
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Alpha-D-Glucose pentaacetate CAS:3891-59-6
Alpha-D-glucose pentaacetate o se vailaʻau vailaʻau e maua mai i le acetylating o vaega hydroxyl o le alpha-D-glucose ma vaega acetyl e lima.E masani ona faʻaaogaina i le faʻaogaina o meaola e avea o se vaega puipuia mo vaega hydroxyl o loʻo iai i gaʻo.E mafai foʻi ona faʻaaogaina e fai ma faʻamatalaga faʻapitoa i suʻesuʻega kemisi ma auʻiliʻiliga, ma avea ma mea amata mo le faʻapipiʻiina o mea eseese.E le gata i lea, o le kulukose pentaacetate ua suʻesuʻeina mo lona faʻaogaina i totonu o le faʻaogaina o vailaʻau, ona o ana meatotino faʻasaʻo.
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AMPD CAS:115-69-5 Tau Faumea
2-Amino-2-methyl-1,3-propanediol, e taʻua foi o le AMPD poʻo le α-methyl serinol, o se vailaʻau vailaʻau faʻatasi ma le faʻasologa mole C4H11NO2.O se amino ava malosi e masani ona faʻaaogaina e avea o se vailaʻau vavalo i le tuʻufaʻatasiga o vailaʻau ma vailaʻau faʻalaʻau.AMPD ua lauiloa mo lona gafatia e galue o se fesoasoani chiral i asymmetric tali, ma taua i le gaosiga o enantiomerically mama tuufaatasiga.E le gata i lea, ua faʻaaogaina e fai ma mea e faʻaogaina i le tausiga o le tagata lava ia ma mea faʻalelei mo ona mea faʻasusu.
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CAPS SODIUM SALT CAS:105140-23-6
CAPS sodium salt ose zwitterionic buffer e masani ona fa'aogaina i fa'aoga o meaola fa'aola ma mole mole.O lo'o iai lona tau pKa e tusa ma le 10.4, e mafai ai ona aoga mo va'aiga pH i le va o le 9.7 ma le 11.1.CAPS sodium masima o lo'o fa'aaogaina i le porotini electrophoresis, fa'agaioiga enzymatic, su'esu'ega o meaola ma vaila'au, ma fa'asalalauga tu'ufa'atasiga.E maua ai le tetee i suiga o le pH e mafua mai i mea leaga ma e lelei le soluble i le vai.
