Tris(hydroxymethyl)nitromethane CAS:126-11-4

Tris (hydroxymethyl) nitromethane, e masani ona taʻua o Tris poʻo THN, o se vailaʻau faʻamaʻi ma le fua mole C4H11NO4.Ose ma'a tioata samasama samasama e sili ona so'u ile vai.Tris o loʻo faʻaaogaina lautele e fai ma sui faʻapipiʻi i faʻaoga o meaola faʻapitoa ma molecular biology.E fesoasoani i le faatumauina o se tulaga mautu o le pH i vaifofo, e taua tele mo auala eseese e pei ole DNA ma le RNA vavae ese, PCR, gel electrophoresis, faʻamamaina o porotini, aganuʻu sela, kemisi porotini, enzymology, ma suʻesuʻega o meaola.O mea fa'apolopolo a Tris e mafai ai ona maua tulaga sili ona lelei i nei su'esu'ega, fa'amautinoa i'uga sa'o ma fa'atuatuaina.

Poloaiga po'o Talosaga Fa'amatalaga

Fa'amatalaga Oloa

Faailoga o oloa

Fa'aoga ma A'afiaga

Aafiaga:

Malosiaga Puipuia: E galue Tris e avea o se sui puipuia lelei ona o lona gafatia e talia pe foaʻi protons, faʻamautu le tulaga mautu pH i fofo.E masani ona faʻaaogaina e avea o se vaega muamua i faiga faʻapipiʻi e faʻamautu ai le pH o meaola faʻataʻitaʻiga ma gaioiga.

Talosaga:

Molecular Biology: Tris o loʻo faʻaaogaina lautele e fai ma sui faʻapipiʻi i auala eseese o meaola mole mole, e aofia ai le DNA ma le RNA vavae ese, PCR, gel electrophoresis, ma le faʻamamaina o porotini.E fesoasoani i le faatumauina o se siosiomaga mautu o le pH, e mafai ai ona sili atu tulaga mo gaioiga enzymatic ma fegalegaleaiga mole.

Aganu'u Cell: E masani ona fa'aogaina Tris i fa'asalalauga tu'ufa'atasiga e fa'atumauina ai pea le pH ma le paleni osmotic, e lagolagoina ai le tuputupu a'e o le sela soifua maloloina ma le ola.

Polotini Chemistry: E fa'aaogaina le Tris i su'esu'ega kemisi porotini, e pei o le solubilization o porotini, su'esu'ega mautu o porotini, ma su'esu'ega fa'amauina o le polotini-ligand.E fesoasoani e fa'atumauina le tulaga o le pH mana'omia, fa'amautinoa le gaugau ma le gaioiina lelei o porotini.

Enzymology: Tris o loʻo faʻafaigaluegaina i suʻega faʻamaʻi eseese e faʻamalieina ai tulaga pH e manaʻomia mo le gaioiga faʻamalosi.E fa'amautinoaina fa'atuatuaina ma toe fa'afo'i fa'ai'uga, e mafai ai ona fua sa'o ole enzyme kinetics ma su'esu'ega fa'asaina.

Su'esu'ega Fa'aolaola: E fa'aaogaina le Tris e fai ma vaega i le tele o su'esu'ega fa'aola kemikolo ona o ona mea fa'apolopolo.E fa'atumauina pea le pH i taimi o su'esu'ega colorimetric, spectrophotometric, ma enzymatic, fa'aleleia le sa'o ma le fa'amaoni o taunu'uga.