5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide o se faʻaogaina o loʻo faʻaaogaina i suʻesuʻega faʻapitoa o meaola, aemaise lava mo le suʻesuʻeina ma le vaʻaia o le gaioiga o le enzyme.O se mea'ai e mafai ona fa'afefeteina e enzymes fa'apitoa, e mafua ai le tu'uina atu o se mea lanu po'o se mea fa'alelei.
O lenei tuufaatasiga e masani ona faʻaaogaina i suʻega e iloa ai le i ai ma le gaioiga o enzymes pei ole beta-galactosidase ma beta-glucuronidase.O nei enzymes e faʻapipiʻiina le acetyl ma le glucosaminide vaega mai le meaʻai, e oʻo atu ai i le faʻavaeina o se chromophore lanumoana pe lanumeamata.
O le fausaga tulaga ese o le 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide e mafai ai ona faigofie ona iloa ma faʻatusatusaina le gaioiga o le enzyme.O lona fa'aogaina i metotia fa'ata'ita'i eseese, e aofia ai le histochemistry, immunohistochemistry, ma su'esu'ega e fa'atatau i sela, ua saofagā i le malamalama atili i galuega ole enzyme.