Disodium 4-nitrophenylphosphate CAS:4264-83-9

O le aafiaga autū ma le faʻaaogaina o le disodium 4-nitrophenylphosphate o lona faʻaaogaina lea o se substrate chromogenic mo suʻega o le enzyme alkaline phosphatase. A faʻafefiloi lenei vailaʻau ma le alkaline phosphatase, e vavaeʻeseina e le enzyme le vaega phosphate mai le disodium 4-nitrophenylphosphate, ma iʻu ai i le fausiaina o le 4-nitrophenol.

O le 4-nitrophenol ua gaosia o se vailaʻau lanu samasama e mafai ona iloa i le spectrophotometrically i le 405 nm. O lenei suiga o le lanu e faʻaaogaina e fuaina ai le gaioiga o le alkaline phosphatase i faʻataʻitaʻiga, e pei o vai ola poʻo cell lysates.

O le fa'aogaina o le disodium 4-nitrophenylphosphate i su'ega o le enzyme e maua ai se metotia faigofie mo le fuaina o le gaioiga o le alkaline phosphatase. O lenei enzyme e a'afia i le tele o faiga fa'aola ma e masani ona fa'aaogaina o se fa'ailoga mo le eseesega o a'ano, galuega a le ate, ma le metabolism o ponaivi.

E le gata i lea, e mafai ona faʻaaogaina le disodium 4-nitrophenylphosphate o se substrate mo isi phosphatases, e pei o le acid phosphatase poʻo le protein phosphatases, e fuafua i le faʻatulagaga faʻataʻitaʻi ma le sini o suʻesuʻega.